... v) The optimised RT-PCR conditions (final concentrations in 50 µl total volume) for detection of TSV in shrimp tissue samples are: primers (0.46 µM each), dNTPs (300 µM each), rTth DNA polymerase (2.5 U/50 µl), manganese acetate (2.5 mM), in...
http://www.oie.int/eng/normes/fmanual/A_00048.htm